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KMID : 1134819970260061237
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 6 p.1237 ~ p.1245
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Morphological and Physiological Properties of Interspecific Electrofusants, Bacteriocin Producer, from Lactobacillus sp. JC - 7 and Lactobacillus acidophilus 88
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Jo Young-Bae
Choi Hyun-Jung
Bae Kyung-Mi
Jun Hong-Ki
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Abstract
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Interspecific fusants were made from the cells of two strains of Lactobacillus genus, a streptomycin resistant Lactobacillus sp. JC-7 and a kanamycin resistant L. acidophilus 88. The morphological and physiological properties of the fusants were examined by determining bacteriocin productivity, acid-producing activity, ability of carbohydrates utilization and three important enzyme activities. The fusants produced a bacteriocin against indicator strains and fusant No.1, 4 exhibited a larger inhibition zone compared to that of L. acidophilus 88. β-Galactosidase, phospho-β-galactosidase, lipase activities and resistance to NaCl of Lactobacillus sp. JC-7 were better than those of L. acidophilus 88. Fusant No. 3 and 7 exhibited excellent lipase activities. Protease activity and acid productivity of L. acidophilus 88 were better than those of Lactobacillus sp. JC-7. Protease activities of all fusants were higher than those of parental strains, and especially fusant No. 5 and 7 exhibited excellent proteolysis ability.
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KEYWORD
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Lactobacillus sp. JC-7, Lactobacillus acidophilus, electrofusion, recombinant strain
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